Antigen

Antigen Class

TFs and others

Antigen

HAND2

Cell type

Cell type Class

Neural

Cell type

IMR-32

Primary Tissue

Brain

Tissue Diagnosis

Neuroblastoma

Sample

source_name

IMR32_HAND2_ChIPSeq

cell line

IMR32

cell type

Neuroblastoma

chip antibody

HAND2 (Abcam ab204000)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31892173

Reads aligned (%)

92.8

Duplicates removed (%)

18.3

Number of peaks

19734 (qval < 1E-05)

hg19

Number of total reads

31892173

Reads aligned (%)

92.4

Duplicates removed (%)

19.0

Number of peaks

19654 (qval < 1E-05)