Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HAND2

Cell type

Cell type Class
Neural
Cell type
IMR-32
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
IMR32_HAND2_ChIPSeq
cell line
IMR32
cell type
Neuroblastoma
chip antibody
HAND2 (Abcam ab204000)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody following the Active Motif's instructions of the ChIP-IT High Sensitivity Kit. Libraries were prepared according to Illumina's TruSeq ChIP Sample Preparation Guide.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
31892173
Reads aligned (%)
92.8
Duplicates removed (%)
18.3
Number of peaks
19734 (qval < 1E-05)

hg19

Number of total reads
31892173
Reads aligned (%)
92.4
Duplicates removed (%)
19.0
Number of peaks
19654 (qval < 1E-05)

Base call quality data from DBCLS SRA